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Butanedione monoxime increases the viability and yield of adult cardiomyocytes in primary cultures

The myosin ATPase inhibitor BDM modulates ANP gene expression and confers cytoprotection in cultures of adult cardiomyocytes (Arbeitstitel)
: Thum, T.; Borlak, J.


Cardiovascular toxicology 1 (2001), Nr.1, S.61-72
ISSN: 1530-7905
Fraunhofer ITA ( ITEM) ()
BDM; cytoprotection; atrial natriuretic peptide; heart cells; cell culture; calcium; enzyme; kinetics; gene expression

Various protocols for the isolation and cultivation of adult rat cardiomyocytes were compared, and the cytoprotective potential of the reversible myosin ATPase inhibitor butanedione monoxime (BDM) was evaluated based on cell yield, cell vitality, lactate dehydrogenase (LDH) and creatine kinase (CK) release, and the mRNA expression of atrial natriuretic peptide (ANP). Overall, a yield of 11.9 x 106 cells with >92% cell vitality was obtained when BDM was added to the isolation and cultivation buffers. In contrast, cell vitality ranged from 30% to 70% and cell yield was (4
The results obtained with BDM warrant further investigation into its cytoprotective potential during ischemia and damage to the cytoskeleton.