Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Impact of magnetic labeling on human and mouse stem cells and their long-term magnetic resonance tracking in a rat model of parkinson disease

 
: Stroh, A.; Boltze, J.; Sieland, K.; Hild, K.; Gutzeit, C.; Jung, T.; Kressel, J.; Hau, S.; Reich, D.; Grune, T.; Zimmer, C.

:

Molecular imaging 8 (2009), Nr.3, S.166-178
ISSN: 1535-3508
ISSN: 1536-0121
Englisch
Zeitschriftenaufsatz
Fraunhofer IZI ()
iron-oxide particle; neural precursor cell; in-vivo; oxidative stress; transfection agent; alzheimers-disease; intracellular-distribution; oxidized protein; bone-marrow; Transplantation

Abstract
Magnetic resonance imaging (MRI) of magnetically labeled stem cells has become a valuable tool in the understanding and evaluation of experimental stem cell-based therapies of degenerative central nervous system disorders. This comprehensive study assesses the impact of magnetic labeling of both human and rodent stem cell-containing populations on multiple biologic parameters as maintenance of sternness and oxidative stress levels. Cells were efficiently magnetically labeled with very small superparamagnetic iron oxide particles. Only under the condition of tailored labeling strategies can the impact of magnetic labeling on vitality, proliferation, pluripotency, and oxidative stress levels be minimized. In a rat model of Parkinson disease, magnetically labeled mouse embryonic stem cells were tracked by high-field MRI for 6 months. Significant interindividual differences concerning the spatial distribution of cells became evident. Histologically, transplanted green fluorescent protein-positive iron oxide-labeled cells were clearly identified. No significant increase in oxidative stress levels at the implantation site and no secondary uptake of magnetic label by host phagocytotic cells were observed. Our study strongly suggests that molecular MRI approaches must be carefully tailored to the respective cell population to exert minimal physiologic impact, ensuring the feasibility of this imaging approach for clinical applications.

: http://publica.fraunhofer.de/dokumente/N-101731.html