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Verbesserte strukturiert-funktionale Bindematrices fuer Biomolekuele

Functional element, useful e.g. for analyte detection and controlling cell growth, comprises nanoparticles immobilized on a surface and having specific-binding properties.
: Weber, A.; Schiestel, T.; Tovar, G.; Brunner, H.

Frontpage ()

DE 2001-10164309 A: 20011228
DE 2001-10164309 A: 20011228
WO 2002-EP14769 A: 20021227
DE 10164309 A1: 20030710
Patent, Electronic Publication
Fraunhofer IGB ()

Die vorliegende Erfindung betrifft Funktionselemente, die auf einem Traeger angeordnete, biofunktionalisierte Nanopartikel enthaltende Mikrostrukturen umfassen, Verfahren zur Herstellung dieser Funktionselemente sowie die Verwendung derselben.


WO2003056336 A UPAB: 20030813 NOVELTY - Functional element (A) comprises a carrier having a surface and at least one microstructure (MS), on the surface, where MS consists of individual nanoparticles (NP) which exhibit molecule-specific recognition sites (X) that make MS addressable. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparation of (A) by applying to the carrier surface first a layer of at least one binding agent and then NP. USE - (A) are useful: (i) in detection processes (mass spectrometry, fluorescence or UV/vis spectrometry, fluorescent or light microscopy, wave-guide or impedance spectrometry and other electrical methods); (ii) for controlling adhesion and growth of cells; (iii) for detection/isolation of biomolecules, e.g. interacting proteins; (iv) for development of pharmaceutical preparations, and for assessing their effects and side-effects; (v) for diagnosis of diseases, e.g. identification of pathogens or mutated genes, in humans or animals; (vi) to analyze microbial contamination of samples (water, soil, food and animal fodder); and (vii) as electrical components in a biocomputer. ADVANTAGE - In (A), biomolecules are immobilized with retention of activity and at high density. NP are morphologically and chemically stable for a long time in solution; provide a very high surface area:volume ratio and make possible a degree of miniaturization not previously achieved.