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Cooperative effects of colony-stimulating factor 1 and recombinant interleukin 2 on profileration and induction of cytotoxicity of macrophage precursors generated from mouse bone marrow cell cultures

: Schwinzer, R.; Lohmann-Matthes, M.-L.; Baccarini, M.; Li, H.

Journal of Experimental Medicine 169 (1989), No.3, pp.973-986 : Abb.,Lit.
ISSN: 0022-1007
ISSN: 1540-9538
Journal Article
Fraunhofer ITA ( ITEM) ()
bone marrow cell culture; cell surface marker expression; colony stimulating factor; CSF 1; cytotoxicity; IL2; interleukin-2; macrophage precursor cell; mouse; proliferation

Precursor cells for NK activity, present in the light fraction of fresh mouse bone marrow, were cultivated in vitro in the presence of either CSF-1, IL-2, or a combination of factors. IN the presence of only CSF-1, strong proliferation was induced. Cells quickly passed the macrophage precursor stage and matured to typical macrophages. Neither granula formation nor NK activity were induced. Under culture conditions with only IL-2 NK activity had developed after 3 d, however, no significant proliferation occurred. In the presence of both factors strong proliferation was induced, and concomitantly, granula formation and NK activity developed. Apparently, proliferation depeneded on CSF-1 and granula formation, and NK cytotoxicity was induced by IL-2. When proliferating cells with strong anti-YAC-1 activity from a culture in CSF-1 plus IL-2 were further culivated in only IL-2, the content of granula further increased, whereas proliferation gradually stopped. In contrast, when these cells f rom CSF-1 plus IL-2 culture were further cultivated in only CSF-1, granula disappeared and NK activity was lost, whereas sustained proliferation and differentiation to macrophages occurred. Only under culture conditions with both factors were proliferation and NK activity both maintained. More than 90% of cells from a 3-d culture in CSF-1 plus IL-2 expressed the NK 1.1. marker, whereas F4/80 was only marginally detected by FACS analysis. After two further days in culture, 70% of the cells expressed F4/80 and 60% coexpressed NK 1.1. and F4/80.