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Nanopartikel mit daran immobilisiertem biologisch aktivem TNF

Nanoparticles that carry tumor necrosis factor-family proteins, useful e.g. for treatment and diagnosis of tumors, retain biological activity of the protein.
: Tovar, G.; Brunner, H.; Schiestel, T.; Pfizenmaier, K.; Grell, M.; Scheurich, P.; Hammer, A.

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DE 2001-10144252 A: 20010831
DE 2001-10144252 A: 20010831
WO 2002-EP9185 A: 20020816
DE 10144252 A1: 20030327
Patent, Electronic Publication
Fraunhofer IGB ()

Die vorliegende Erfindung betrifft Nanopartikel mit daran immobilisiertem Tumor-Nekrose-Faktgor (TNF), wobei TNF in Form eines Trimers gerichtet und unter Beibehaltung seiner biologischen Aktivitaet am Traeger immobilisiert ist, Verfahren zur gerichteten Immobilisierung von TNF an Nanopartikeln, die Verwendung der immobilisierten TNF aufweisenden Nanopartikel zur Identifizierung und/oder Isolierung von TNF-Bindungspartnern sowie zur Identifizierung und/oder Isolierung von Hemmstoffen der Interaktion zwischen TNF und seinen Bindungspartnern, die Verwendung derartiger Naopartikel zur Herstellung einer pharmazeutischen Zusammensetzung, insbesondere zur Therapie von Tumoren, sowie pharmazeutische und diagnostische Zusammensetzungen, die derartige Nanopartikel enthalten.


WO2003020320 A UPAB: 20030526 NOVELTY - Nanoparticles (NP) comprise a carrier having: (1) a surface with functional groups (1A) and (2) at least one monomer of a protein (I) of the tumor necrosis factor (TNF) family having a functional group (2A) complementary to 1A and linked to the carrier through 1A/2A. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for the following: (1) method for directed immobilization of (I) on an NP with retention of biological activity; and (2) method for identifying and/or detecting agents that modulate (particularly prevent or inhibit) interaction between immobilized (I) and its complementary receptor. ACTIVITY - Cytostatic; Antiinflammatory; Immunosuppressive; Vulnerary. MECHANISM OF ACTION - TNF protein function Modulator . USE - NP are used: (i) to identify/detect agents, potentially useful therapeutically, that modify the interaction between (I) and its receptors; (ii) to identify and isolate binding partners of (I); (iii) as pharmaceuticals for treatment of tumors, inflammation, immune system disorders, wound healing and to modify angiogenesis; and (iv) for in vitro diagnosis, e.g. to detect or label particular cell types, particularly to isolate such cells (including removal of tumor cells in an extracorporeal system) or to induce reactions (differentiation, proliferation and apoptosis) mediated by such cells. ADVANTAGE - (I) are immobilized without loss of biological activity.