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Chemoselective nitro group reduction and reductive dechlorination initiate degradation of 2-chloro-5-nitrophenol by Ralstonia eutropha JMP134

 
: Schenzle, A.; Lenke, H.; Spain, J.C.; Knackmuss, H.-J.

Applied and environmental microbiology 65 (1999), No.6, pp.2317-2323 : Ill., Lit.
ISSN: 0099-2240
English
Journal Article
Fraunhofer IGB ()
2-chloro-5-nitrophenol degradation; biodegradation; nitro group reduction; Ralstonia eutropha JMP134; reductive dechlorination

Abstract
Ralstonia eutropha JMP134 utilizes 2-chloro-5-nitrophenol as a sole source of nitrogen, carbon, and energy. The initial steps for degradation of 2-chloro-5-nitrophenol are analogous to those of 3-nitrophenol degradation in R. eutropha JMP134. 2-Chloro-5-nitrophenol is initially reduced to 2-chloro-5-hydroxylaminophenol, which is subject to an enzymatic Bamberger rearrangement yielding 2-amino-5-chlorohydroquinone. The chlorine of 2-amino-5-chlorohydroquinone is removed by a reductive mechanism, and aminohydroquinone is formed. 2-Chloro-5-nitrophenol and 3-nitrophenol induce the expression of 3-nitrophenol nitroreductase, of 3-hydroxylaminophenol mutase, and of the dechlorinating activity. 3-Nitrophenol nitroreductase catalyzes chemoselective reduction of aromatic nitro groups to hydroxylamino groups in the presence of NADPH. 3-Nitrophenol nitroreductase is active with a variety of mono-, di-, and trinitroaromatic compounds, demonstrating a relaxed substrate specificity of the enzyme. N itrosobenzene serves as a substrate for the enzyme and is converted faster than nitrobenzene.

: http://publica.fraunhofer.de/documents/PX-7688.html