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  4. Changes in light-scatter profile, membrane depolarization and calcium mobilization of neutrophils induced by G-CSF in vivo
 
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1994
Journal Article
Title

Changes in light-scatter profile, membrane depolarization and calcium mobilization of neutrophils induced by G-CSF in vivo

Abstract
To extend our studies about phenotypical and functional alterations of G-CSF-induced neutrophils we have evaluated their light-scatter profile, mobilization of intracellular calcium ([Ca2+]i) and membrane depolarization after stimulation. A significant increase in the forward scatter signals could be demonstrated in such neutrophils from patients with neutropenias of various origin and from healthy test subjects. This increase began 4 h and returned to normal 96 h after G-CSF injections in the latter group. We found an impairment of [Ca2+]i mobilization in neutrophils from patients with glycogen storage disease type IB after stimulation of these cells with fMLP. It was even more pronounced than in severe congenital neutropenia (SCN). However, [CA2+]i fluxes were normal when ionomycin was used. Neutrophils from patients with cyclic neutropenia (cyNP) and chemotherapy-induced neutropenia (chNP) mobilized [CA2+]i similar to those from healthy donors. (abstract truncated)
Author(s)
Spiekermann, K.
Emmendörffer, A.
Elsner, J.
Raeder, E.
Lohmann-Matthes, M.-L.
Roesler, J.
Welte, K.
Journal
British Journal of Haematology  
DOI
10.1111/j.1365-2141.1994.tb05066.x
Language
English
ITA  
Keyword(s)
  • granulocytes

  • immunology

  • neutropenia

  • neutrophils

  • signal transduction

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