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Verfahren zum Nachweis eines Analyten in einem Probentraeger und entsprechende Vorrichtung

Detection of an analyte in a sample vessel comprises exposing the sample to a light beam, and measuring intensity according to the interface geometry.
: Mueller, M.; Brunner, H.; Vitzthum, F.; Bernhagen, J.

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DE 2000-10047007 A: 20000922
DE 2000-10047007 A: 20000922
WO 2001-EP10389 A: 20010908
DE 10047007 C2: 20020829
Patent, Electronic Publication
Fraunhofer IGB ()

Das Verfahren und die Vorrichtung dienen zum Nachweis eines Analyten in einem Probentraeger (PT), wobei der Analyt gegebenenfalls in und/oder an einem fluessigen, gasfoermigen oder festen Aufnahmemedium enthalten ist unter Ausbildung mindestens einer Fluessigkeit. Hierbei ist vorgesehen, dass die geometrische Ausgestaltung einer Grenzflaeche der Fluessigkeit zum Nachweis des Analyten im Probentraeger mittels Messung eines von der Gestalt der Grenzflaeche abhaengigen Messwerts herangezogen wird.


WO 200225245 A UPAB: 20020610 NOVELTY - Detecting the presence of an analyte in a sample vessel, contained in and/or at a liquid or gas take-up medium, comprises the addition of at least one fluid to the vessel. The geometric structure of the fluid interface is measured, and the measurements related to the interface shape register the analyte. DETAILED DESCRIPTION - To detect an analyte in a sample vessel, the analyte or the fluid is exposed to a light beam. The light intensity, through the geometric shape of the interface between the fluid and the analyte take-up, is monitored by a detector unit to register the analyte in the sample vessel. The detector gives a photometric measurement or a luminometric measurement of the light intensity. The detector is moved to alter the gap at the interface between the fluid and the analyte take-up. An optical lens system between the interface and the detector optimizes the light intensity measurements. A qualitative and/or quantitative detection of the analyte uses suitable calibration series. The light source is moved to shift its light beam to increase measurement sensitivity. The sample vessel and/or the fluid is subjected to a magnetic or electromagnetic field to optimize the measurement. An INDEPENDENT CLAIM is included for an apparatus to detect an analyte in a sample vessel (PT). A light source (LQ) delivers a light beam through the fluid in the vessel and the interface (22,23) between the fluid and the analyte take-up. A detector (D) registers the light intensity according to the geometric shape of the interface. Preferred Features: The detector unit has a curved detection surface. A lens system is between the detector unit and the sample carrier. The detector unit has at least one frontal detection surface in relation to the sample carrier and/or side detection sections. USE - The technique for the detection of analytes covers the bonding and/or reaction of components of gases and/or fluids with interface partners. It can be used for special enzyme catalyst reactions occurring in fluids, surfaces or interface surface. Two or more fluids of different densities can be used, also a gas with a multi-component system. It can also be used for interaction at interface surfaces between fluid/fluid, fluid/solid, fluid/gas, and solid/gas combinations. It can also be used for enzymes with interface activity, e.g. lipases. It is also useful for interface polymerizations. ADVANTAGE - The system gives a low-cost and accurate analyte detection operation, without using expensive and toxic materials. It can use a sample vessel of small dimensions, and has fast measurement times. It can be used in paralleling and automation systems, and is especially useful for bio-chips and lab-on-a-chip, and in low sample and high sample throughput applications.