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Biotechnologisch interessante Proteine aus Katzenhaien

New dihydroliponamide dehydrogenase from dogfish, useful as diaphorase for converting substrates to dye, in analysis, and for producing enantiomerically pure lipoic acid.
 
: Vitzthum, F.; Bernhagen, J.; Bisswanger, H.; Brunner, H.

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DE 2000-10007531 A: 20000218
DE 2000-10007531 A: 20000218
WO 2001-EP1632 A: 20010214
DE 10007531 C2: 20031224
C12N0009
German
Patent, Electronic Publication
Fraunhofer IGB ()

Abstract
Die vorliegende Erfindung betrifft ein Protein mit der Aktivitaet einer Dihydroliponamid-Dehydrogenase aus Katzenhaien sowie Verfahren desselben und Verwendungen derselben.

 

WO 200160988 A UPAB: 20011105 NOVELTY - Protein (I) with the activity of a dihydroliponamide dehydrogenase is a homodimer of relative molecular weight 100-150 kDa (gel filtration) and relative molecular weight of the subunits 50 kDa (sodium dodecylsulfate polyacrylamide gel electrophoresis), is new. DETAILED DESCRIPTION - Protein (I) with the activity of a dihydroliponamide dehydrogenase is a homodimer of relative molecular weight 100-150 kDa (gel filtration) and relative molecular weight of the subunits 50 kDa (sodium dodecylsulfate polyacrylamide gel electrophoresis), is new. (I) is prepared by: (a) producing a crude extract of muscle tissue from the dogfish Scyliorhinus canicula; (b) gel filtration through a Superdex (RTM) column; and (c) anion-exchange chromatography. An INDEPENDENT CLAIM is also included for preparation of (I). ACTIVITY - Cytostatic; anti-HIV (human immunodeficiency virus); antidiabetic; heptatropic; antiarteriosclerotic. No biological data is given. MECHANISM OF ACTION - None given. USE - (I), and related enzymes from other fish of the family Scyliorhinididae, are used (claimed) as diaphorase, particularly for oxidative or reductive conversion of a substrate to a dye, i.e. for (in)direct detection (in the visible spectrum) of substances such as amino acids, proteins, nucleic acids, and co-factors, in coupled enzyme substrate tests (claimed). Other uses for (I) are in synthesis of enatiomerically pure (or enriched) lipoic acid, particularly in the (R) conformation, useful as e.g. radical scavenger, antioxidant (regeneration of Vitamin C and potentiation of Vitamin E activity), protection of membranes against lipid peroxidation, or of nucleic acids or proteins, e.g. for prevention and treatment of cancer, acquired immune deficiency syndrome, diabetes, liver cirrhosis, polyneuritis, arteriosclerosis, lactate acidosis and heavy metal poisoning. ADVANTAGE - (I) has a low temperature maximum and high specificity at low temperature, so catalyzes reactions rapidly at room temperature, eliminating the need for, and expense of, thermostatting. It has relatively good temperature stability (allowing long-term storage at ambient temperature) and its enzymatic activity can be reversed by altering the temperature (functioning as temperature switch), i.e. increasing the temperature to 70 deg. C suppresses activity but activity is restored on cooling.

: http://publica.fraunhofer.de/documents/PX-69823.html