Production of isoprene comprises culturing a microorganism containing a plant isoprene synthase gene in a container at a temperature above 34 deg. C and capturing gaseous isoprene from the waste air. USE - Isoprene is used in the plastics and rubber industries; it is used to make isoprene rubber for the tyre industry; it is used to make butyl rubber for the manufacture of seals and hoses; it is used to make isoprenoids for use in the perfume industry.