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VERFAHREN UND VORRICHTUNG ZUM AUFSCHLUSS BIOLOGISCHER ZELLEN ZUR EXTRAKTION UND ANALYSE DER ZELLINHALTE

Extraction of proteins and nucleic acids from biological cells .
 

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DE 1997-19752961 A: 19971128
DE 1997-19752961 A: 19971128
DE 19752961 C1: 19990715
C12N0013
German
Patent, Electronic Publication
Fraunhofer IPA ()

Abstract
NOVELTY - Biological cells are broken down to extract the cell contents for analysis by being subjected to an electric field. The cells are brought into contact with a substance to encourage cell breakdown, before or while the electrical field affects the cells. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for an apparatus to take the cells suspended in a medium, in tissue form, or on a carrier as a sample. It has two flat and facing electrodes. The cover (4) has a convex surface towards the sample zone (1), over it. USE - The method is especially for the extraction of proteins and nucleic acids (DNA and RNA) from biological cells, such as for medical diagnosis, and for subsequent polymerase chain reactions (PCR). ADVANTAGE - The process is automated, with a simple apparatus and procedure. It gives a high yield of the cell contents, irrespective of the cell types, in short process times to give an inexpensive and rapid and accurate technique. DESCRIPTION OF DRAWING(S) - The drawing shows a cross section of the apparatus. sample zone 1 lower electrode 3 upper electrode cover 4

: http://publica.fraunhofer.de/documents/PX-38494.html