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Susceptibility of 3H-thymidine-prelabelled Leishmania donovani amastigotes and promastigotes to the cytotoxic activity of peritoneal splenic and liver macrophages

: Emmendörffer, A.; Hockertz, S.; Lohmann-Matthes, M.-L.

Immunobiology 179 (1989), No.2/3, pp.202-213
ISSN: 0171-2985
Journal Article
Fraunhofer ITA ( ITEM) ()
3H-thymidine; amastigote; Leishmania donovani; Leishmaniasis; liver; macrophage; mouse; parasite; phagocytosis; promastigote; protozoan parasite; spleen

C57BL/6 macrophage populations from spleen and liver, the main organs for the manifestation of visceral leishmaniasis, were investigated for their ability to perform spontaneous phagocytosis-associated killing of 3H-thymidine pelabelled L. donovani amastigotes and promastigotes. The results showed that organ macrophages from spleen and liver killed L. donovani amastigotes and promastigotes spontaneously with high efficiency. This type of killing was strongly enhanced when spleen and liver macrophages were activated. This phagocytosis-associated killing mechanisms may contribute, to a large extent, in maintaining the infection under control in vivo, by drastically reducing the amount of parasites that is required to establish intracellular parasitism. To be able to assay phagocytosis-associated destruction of both promastigotes and amastigotes, a reproducible system for the production in vitro of L. donovani amastigotes by the macrophage call line J774 was developed. The DNA of the Leis hmania amastigotes was labelled with 3H-thymidine with high efficiency. The spontaneous label release of prelabelled L. donovani amastigotes was comparable to that of prelabelled promastigotes over an assay period of 24 hours.