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Removal of 3' nontranslated cDNA sequence improves interferon-gamma synthesis in Escherichia coli.

Entfernen der 3' nichttranslatierten cDNS-Sequenz erhöht die Synthese von Interferon-gama in Escherichia coli
: Böhm, J.; Krehle, K.; Otto, B.

Arzneimittel-Forschung 41 (1991), No.2, pp.1306-1309
ISSN: 0004-4172
Journal Article
Fraunhofer ITA ( ITEM) ()
Escherichia coli; gene; human interferon-gamma; interferon-gamma; pharmaceutic; protein; recombinant interferon-gamma

The cDNA coding for human interferon Gamma contains approximately 600 basepairs not translated sequence with 4 adenine/thymidine (AT)-rich clusters at its 3'end. The influence of this sequence on the expression of recombinant human Interferon Gamma in Escherichia coli was investigated. Removal of the non-translated sequence and especially of the AT-rich clusters yielded an increase in expression of interferon Gamma from 10 to 40% of total protein. These results may also be important for the production of other recombinant human proteins with similar 3'sequences in their cDNA.