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Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten. Quantitative evaluation of thin-layer chromatograms by means of the solvatochromic fluorescence of 8-phenylamino-1-naphthalenesulfonate
Quantitative Auswertung von Dünnschicht-Chromatogrammen mit Hilfe der solvatochromen Fluorescenz von 8-Phenylamino-1-naphthalin-Sulfonat
| Fresenius' Zeitschrift für Analytische Chemie 334 (1989), pp.31-33 : Abb.,Tab.,Lit.
ISSN: 0016-1152
ISSN: 0372-7920 |
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| English |
| Journal Article |
| Fraunhofer IUCT ( IME) () |
| Dünnschichtchromatogram; Fluoreszenz; Quantifizierung; Solvatochromie |
Abstract
Polarity dependent fluorescence of 8-(phenyl-amino)-1-naphtalene sulfonate (8.1 ANS) was used to develop a quantitative evaluation method for thin-layer chromatograms (TLCs). After the compounds have been separated the dye is applied to the chromatogram where it is extracted by the substances and shows - upon exitation - a significant fluorescence. This is a way to quantify photophysically inactive molecules without preceding derivatization. Fluorescence intensity correlate well with the amount of the compounds as is shown in the cases of three wax components.