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1999
Journal Article
Titel
Optimizing expression of a rare codon-rich viral protein in Escherichia coli using the IMPACT system
Abstract
The present data demonstrate that fusion to a C-terminal affinity purification tag using the IMAPCTĂ– system is a suitable method for expression of intact and soluble NSM protein in E. coli. This result suggests that linkage to C-terminal fusion partners has an advantage over linkage to N-terminal fusions for E. coli expression and purification of rare codon rich functional heterologous proteins.