Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the C14Co5 deletion in albino mutant mice

 
: Carnwath, J.W.; Kaliner, B.; Paul, D.; Rinchik, E.M.; Tönjes, R.R.; Weith, A.; Winking, H.

Genomics 10 (1991), No.3, pp.686-691
ISSN: 0888-7543
English
Journal Article
Fraunhofer ITA ( ITEM) ()
chromosome 7; chromosome deletion; chromosome mapping; chromosomes; DNA; gene expression regulation; genetic; microclone; mouse; regulator gene

Abstract
A group of radiation-induced perinatal-lethal deletions that include the albino (c) locus on mouse chromosome 7 causes failure of expression of various hepatocyte-specific genes when homozygous. The transcription of such genes could be controlled in trans by a regulatory gene(s) located within the proximal region of the C14CoS deletion. To identify this potential regulatory gene, a microclone library was established from microdissected D and E bands of chromosome 7. Three nonoverlapping microclones (E305, E336B, and E453B) hybridizing with wildtype but not with C14CoS/C14CoS DNA were isolated. E336B represents a single-copy DNA fragment, whereas E305 and E453B hybridized with 3 and 10 EcoRI DNA restriction fragments, respectively. All fragments map exclusively within the deletion. The microclones hybridized to DNA of viable C6H/C14CoS deletion heterozygotes but not to DNA of homozygotes for the lethal mutation c10R75M, which belongs to the same complementation group as c14CoS. DNA of v iable homozygous mutant C62DSD, which carries a deletion breakpoint proximal to that of c6H, hybridized only with E453B. This microclone identified 6 EcoRI restriction fragments in C62DSD/C62DSD DNA. The results demonstrate that of the isolated microclones, E453B identifies a locus (D7RT453B) that maps closest to the hsdr-1 (hepatocyte-specific developmental regulation) locus, which maps between the proximal breakpoints of deletions c10R75M and c62DSD.

: http://publica.fraunhofer.de/documents/PX-23909.html