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Method for the description of differences in the filamentous structure of the cyctoskeleton in cultured cells

: Kohler, M.; Aufderheide, M.; Ramm, D.


Toxicology letters 72 (1994), No.1/3, pp.33-42
ISSN: 0378-4274
Journal Article
Fraunhofer ITA ( ITEM) ()
animal cell culture; cell; cell culture; cell line; cytochemistry; cytoplasmic filament; cytoskeleton; epithelium; hamster; in vitro; lung; toxicity testing; toxicology; ultrastructure

Over the last few years in vitro cell systems have been established for toxicological investigations. These systems permit the evaluation of effects on the basis of cultured cells in order to replace animal studies. Not only qualitative assessment of cytotoxic effects, but also efforts to quantify these intracellular changes have become more and more important to objectify the results which have been obtained. The cytoskeleton, a dynamic and sensitive system, seems to be a valuable morphological parameter to gain information about the intracellular alterations of drug-influenced cells. Depending on the dose of the substance administered, the cytoskeleton shows morphological alteration in specific components, which fulfil structural as well as metabolic regulatory functions and thus provide information on possible mechanisms. Normally, microtubules as well as the intermediate filament system form 3-dimensional networks.