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Kinetics of transfused neutrophils in peripheral blood and BAL fluid of a patient with variant X-linked chronic granulomatous disease.

Kinetik transfundierter neutrophiler Granulozyten im peripheren Blut und in der BAL-Flüssigkeit eines Patienten mit X-chromosomal vererbter GCD
 

European Journal of Haematology 47 (1991), pp.246-252
ISSN: 0902-4441
English
Journal Article
Fraunhofer ITA ( ITEM) ()
bronchoalveolar lavage; cytochrome B558; dihydrorhodamine 123; flow cytometry; neutrophil; Transfusion; variant X-linked GCD; X-chromosomal vererbte GCD

Abstract
Patients with chronic granulomatous disease (GCD), and uncommon inherited disorder of phagocytes resulting in the defective production of reactive oxygen intermediates, are prone to bacterial and fungal infections. In the case presented, therapeutic efforts including white cell transfusions, and amphotericin B and IFN gamma administration were undertaken to treat pneumonia caused by Aspergillus fumigatus. During a phase of artificial respiration, transfused white cells in peripheral blood and bronchoalveolar lavage fluid were monitored in order to examine their kinetics and functional activity. Using flowcytometrical methods, host-derived and transfused neutrophils could be distinguished by cytochrome b558 expression using the monoclonal antibody 7D5 for immunofluorescent staining as well as by production of reactive oxygen intermediates. Transfused PMN could be detected in both compartments and their kinetics could be followed up to 24 hours after transfusion. Using flowcytometry, eve n small numbers of transfused PM could be measured during episodes of extreme leukocytosis. Since functionally intact transfused PMN were found in the bronchioalveolar lavage fluid, white cell transfusions in combination with antibiotic and immunomodulating therapy should be considered a part of the therapeutic regimens for life-threatening infections in GCD patients.

: http://publica.fraunhofer.de/documents/PX-20459.html