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Influence of interleukin-2 on the differentiation of macrophages

 
: Emmendörffer, A.; Li, H.; Lohmann-Matthes, M.-L.

Pathobiology 59 (1991), No.3, pp.117-121
ISSN: 1015-2008
Annual Conference of the Upper Rhine Universities <1990, Freiburg>
English
Conference Paper
Fraunhofer ITA ( ITEM) ()
colony stimulating factor; CSF; differentiation; IL-2; immunology; Interleukin-2; LAK like-activity; macrophage; macrophage precursor; NK-like activity

Abstract
Macrophage precursor cells, enriched in the light fraction of murine bone marrow, were cultured in vitro under the influence of CSF-1 or IL-2 or both cytokines. In the presence of CSF-1 or CSF-1 and IL-2 strong proliferation occurred, whereas in the presence of only IL-2 or medium, cells did not proliferate. Thus all proliferating cells had CSF-1 receptors and thus belonged to the macrophage lineage. IL-2 induced in these cells the formation of cytoplasmic granules and concomitantly NK-like lytic activity. Under high dosage IL-2 cells further differentiated into cells containing abundant amounts of cytoplasmic granules and exerted LAK type cytotoxicity. When IL-2 was withdrawn from the culture medium, cells could be redirected to develop the properties of typical macrophages when CSF-1 was present. Thus the composition of the cytokines surrounding macrophage precursors decides on their differentiation pathway.

: http://publica.fraunhofer.de/documents/PX-18430.html