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1990
Conference Paper
Titel
In vitro assays for testing the influence of biologically active substances on bone formation
Alternative
In vitro Tests zur Untersuchung des Einflusses biologisch aktiver Substanzen auf Knochenbildung
Abstract
In order to contribute to the understanding of human bone metabolism, two different culture systems have been developed which allow the study of bone formation in vitro: (I) an optimal procedure for human osteoblast-like cell (OLC) culture has been developed which gives cells that exhibit characteristics similar to osteoblasts observed in vivo and therefore is suitable for study human bone formation in vitro, (II) a culture system of muscle cells has been established which allows the detection of proteins which were isolated from bone and dentin and found to be osteogenic in animal experiments. For the fast detection of protein activity, various cell parameters have been adapted. Protein treated myoblasts changed their morphology and cell metabolism. The test-system could also be validated in other cell cultures.