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Heterogeneity in the mobilization of cytoplasmic calcium by human polymorphonuclear leukocytes in response to fMLP, C5a and IL-8/NAP-1

 

Journal of Leukocyte Biology 51 (1992), No.1, pp.77-83
ISSN: 0741-5400
English
Journal Article
Fraunhofer ITA ( ITEM) ()
amines; calcium; complement; cytoplasm; flow cytometry; fluorescence; immunology; interleukins; leukocyte; neutrophils; pharmacology; phenylalanine; spectrum analysis

Abstract
The visible excitation and emission wave-lenghts of the recently developed fluorescent Ca2+ indicator fluo-3 permit analysis of the intracellular Ca2+ concentration, (Ca2+)i, in flow cytometry with a 488-nm argon laser. The role of (Ca2+)i in human polymorphonuclear leukocyte heterogeneity was investigated in response to formyl-methionyl-leucyl-phenylalanine (fMLP), C5a, and interleukin 8/neutropil attractant/activation protein 1 (IL-8/NAP-1) by flow cytometry. The (Ca2+)i changes in different subpopulations within a heterogeneous cell suspension were resolved upon stimulation with fMLP. Using an anti-CD16 phycoerythrin-conjugated antibody and fluo-3 simultaneously, neutrophils affected and nonaffected in Ca2+ mobilization were distinguished in two patients suffering from glycogen storage disease type 1b. In normal neutrophils, a different time course of Ca2+ mobilization of neutrophil subpopulations immdiately after stimulation with fMLP was detected.

: http://publica.fraunhofer.de/documents/PX-16990.html