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  4. Heterogeneity in the mobilization of cytoplasmic calcium by human polymorphonuclear leukocytes in response to fMLP, C5a and IL-8/NAP-1
 
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1992
Journal Article
Title

Heterogeneity in the mobilization of cytoplasmic calcium by human polymorphonuclear leukocytes in response to fMLP, C5a and IL-8/NAP-1

Abstract
The visible excitation and emission wave-lenghts of the recently developed fluorescent Ca2+ indicator fluo-3 permit analysis of the intracellular Ca2+ concentration, (Ca2+)i, in flow cytometry with a 488-nm argon laser. The role of (Ca2+)i in human polymorphonuclear leukocyte heterogeneity was investigated in response to formyl-methionyl-leucyl-phenylalanine (fMLP), C5a, and interleukin 8/neutropil attractant/activation protein 1 (IL-8/NAP-1) by flow cytometry. The (Ca2+)i changes in different subpopulations within a heterogeneous cell suspension were resolved upon stimulation with fMLP. Using an anti-CD16 phycoerythrin-conjugated antibody and fluo-3 simultaneously, neutrophils affected and nonaffected in Ca2+ mobilization were distinguished in two patients suffering from glycogen storage disease type 1b. In normal neutrophils, a different time course of Ca2+ mobilization of neutrophil subpopulations immdiately after stimulation with fMLP was detected.
Author(s)
Elsner, J.
Kaever, V.
Emmendörffer, A.
Breidenbach, T.
Lohmann-Matthes, M.-L.
Roesler, J.
Journal
Journal of Leukocyte Biology  
Language
English
ITA  
Keyword(s)
  • amines

  • calcium

  • complement

  • cytoplasm

  • flow cytometry

  • fluorescence

  • immunology

  • interleukins

  • leukocyte

  • neutrophils

  • pharmacology

  • phenylalanine

  • spectrum analysis

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