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  4. Formation of DNA adducts by the hydroxyanthraquinone lucidin
 
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1991
Conference Paper
Title

Formation of DNA adducts by the hydroxyanthraquinone lucidin

Abstract
Lucidin (1,3-dihydroxy-2-hydroxymethylanthraquinone) is the genotoxic principle of madder roots (Rubia tincterum L.). To investigate whether lucidin, indeed, ineracts covalently with DNA, we incubated the compound (200Myg/ml) for 3 hr with DNA or polydG *polydC and S9 mix and examined the reisolated nucleic acids by 32P-postlabelling. Similar adduct patterns, containing up to 5 spots, were obtained on PEI-cellulose tic with DNA or polydG *polydC. Formation of DNA adducts was also observed after treatment with lucidin (40 Myg/ml) of primary rat hepatocytes in culture. Finally, DNA adducts were detected in liver, kidney, duodenum and colon of male Parkes mice that had been treated orally for 4 days with lucidin (2mg/d), its glycoside lucidinprimeveroside (10mg/d) or Rubia TeephighR (1/2 tablet/d), a pharmaceutical preparation containing the two compounds. These results suggest that the therapeutic use of lucidin may constitute a carcinogenic risk. This work was supported by Deutsche Fors chungsgemeinschaft, Bonn.
Author(s)
Poginsky, B.
Phillips, D.H.
Westendorf, J.
Marquardt, H.
Blömeke, B.
Mainwork
American Association for Cancer Research. Annual Meeting 1991. Proceedings  
Conference
American Association for Cancer Research (Annual Meeting) 1991  
Language
English
ITA  
Keyword(s)
  • anthraquinone

  • carcinogenicity

  • DNA

  • DNA adduct

  • genotoxicity

  • hepatocyte

  • liver

  • lucidin

  • rat

  • rubia tinctorum

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