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  4. Flow cytometry reveals different lag times in rapid cytoplasmic calcium elevations in human neutrophils in response to N-formyl peptide
 
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1993
Journal Article
Title

Flow cytometry reveals different lag times in rapid cytoplasmic calcium elevations in human neutrophils in response to N-formyl peptide

Abstract
Flow cytometric anlyses were performed to study intracellular single-cell calcium transients (Ca2+)i in suspended human neutrophils during the initial phase of N-formyl peptide stimulation. Thereby, two neutrophil populations becam apparent. Early maximally Ca(2+)-responding (high fluorescence) neutrophils and not yet Ca(2+)-responding (low fluorescence) neutropils, but no neutrophils with intermediate levels of (Ca2+)i were detected. Within 7 s the number of low fluorescence neutrophils decreased and the number of high fluorescence neutrophils increased maximally. This suggests that (Ca2+)i transients occured abruptly in individual neutrophils within a time interval below 1 s. At lower N-formyl peptide concentrations the lag times of individual neutrophils and the inverval time of maximal activation of the (Ca2+)i responding neutrophil population increased, however the percentage of (Ca2+)i responding cells decreased.
Author(s)
Elsner, J.
Norgauer, J.
Dobos, G.J.
Emmendörffer, A.
Schopf, E.
Kapp, A.
Roesler, J.
Journal
Journal of Cellular Physiology  
DOI
10.1002/jcp.1041570325
Language
English
ITA  
Keyword(s)
  • calcium

  • cytoplasm

  • flow cytometry

  • neutrophils

  • phenylalanine

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