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A factor induced by differentiation signals in cells of the macrophage lineage binds to the gamma interferon activation site


Molecular and Cellular Biology 14 (1994), No.2, pp.1364-1373
ISSN: 0270-7306
Journal Article
Fraunhofer ITA ( ITEM) ()
cell; cell differentiation; cell line; DNA; genetic transcription; immunoglobulins; interferon; interferon gamma; macrophage; molecular biology; mouse; protein; transcription factor; tyrosine

Rapid transcriptional induction of genes in response to gamma interferon (IFN-gamma) is mediated by the IFN-gamma activation site (GAS) and its cognate protein, the IFN-gamma activation factor (GAF). We describe a GAS-associated, differentiation-induced factor (DIF) as a potential molecular link between the activities of IFN-gamma and of growth and differentiation factors. DIF DNA binding was activated by colony-stimulating factor 1 in murine macrophages and also during tetradecanoyl phorbol acetate-induced differentiation of IFN-gamma treatment in myeloid U937 cells. IFN-gamma activation of DIF decreased significantly upon monocytic differentiation. DIF binding to DNA was inhibited by antiphosphotyrosine antibodies and could be induced by treatment of U937 cells with vanadate. Unlike GAF, DIF-DNA complexes did not contain the 91-kDa protein (p91) from ISGF-3.