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Evidence for the existence of two forms of membrane tumor mecrosis factor. An integral protein and a molecule attached to its receptor

: Decker, T.; Lohmann-Matthes, M.-L.; Lüttig, B.

The Journal of immunology 143 (1989), No.12, pp.4034-4038
ISSN: 0022-1767
ISSN: 1048-3233
ISSN: 1047-7381
ISSN: 1550-6606
Journal Article
Fraunhofer ITA ( ITEM) ()
cytotoxicity; immunology; macrophage; membrane-associated TNF; membrane TNF; membrane tumor necrosis factor; mouse; TNF; tumor necrosis factor

Plasma membranes were isolated from thioglycollate-induced peritoneal mouse macrophages and tested directly in a Cr-release assay against WEHI 164 tumor cells. These membranes showed anti-TNF antibody inhibitable killing of the TNF-sensitive tumor cell line, indicating that membrane-associated TNF is present on mouse macrophages. In order to elucidate whether membrane TNF is an integral protein or a molecule attached to a receptor, cells and plasma membranes were treated with low pH buffer. A partial reduction in TNF activity was observed which could be restored by incubation with exogenous TNF. In a Western blot analysis the integral membrane TNF could be identified as the 26-kDa molecule on activated mouse macrophages. These results indicate that both forms of membrane-associated TNF exist on macrophages and are responsible for cell-mediated cytotoxicity against TNF-alpha-sensitive targets.