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Evaluation of DNA-binding activity of hydroxyanthraquinones occurring in Rubia tinctorum L


Carcinogenesis 12 (1991), No.7, pp.1265-1271 : Abb.
ISSN: 0143-3334
Journal Article
Fraunhofer ITA ( ITEM) ()
alizarin; anthraquinone; Arzneipflanze; DNA; lucidin; Maus; medicinal plant; mouse; mutagenicity; Mutagenität; Pflanzenextrakt; plant extract

The naturally-occurring anthraquinones, alizarin and lucidin, were incubated with DNA in the presence of S9 mix. The isolated DNA was analysed by 32P-postlabelling for the presence of aromatic adducts. Only lucidin formed up to five different DNA adducts in the range from 0.995 to 3.05 adducts/10(8) nucleotides. Lucidin was also incubated with polynucleotides poly(d(A-T)) and polydG *polydC in the presence of S9 mix. Analysis of polydG *polydC revealed a similar adduct pattern to that obtained with lucidin-modified DNA. Alizarin, lucidin, a glycoside mixture containing alizarinprimeveroside and lucidinprimeveroside, and Rubia Teep (a herbal drug made from Rubia tinctorum containing lucidin) were incubated with primary rat hepatocytes for 24 h and the isolated DNA was analysed by 32P-postlabelling. Lucidin, the glycoside mixture and Rubia Teep gave rise to DNA adducts, but alizarin did not. Male Parkes mice were treated orally for 4 days with alizarin (10 mg/d), lucidin (2 mg/d), the gl ycoside mixture (20 mg/d) or Rubia Teep (1/2 tablet/d) and DNA was isolated from liver, kidney, duodenum and colon. Analysis by 32P-postlabelling revealed that lucidin, the glycoside mixture and Rubia Teep, but not alizarin, formed DNA adducts in all the tissues examined but that the adduct patterns were organ-specific.