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Electrode arrays used as transducer and amplifier in biosensing microflow systems


Giber, J.; Göpel, W.; Holmes, I.L.; Horvai, G.; Meixner, H.; Timar-Horvath, V. ; Budapesti Müszaki Egyetem, Eötvös Lorand Fizikai Tarsulat:
Eurosensors VII. Book of Abstracts
Budapest, 1993
Eurosensors <7, 1993, Budapest>
Conference Paper
Fraunhofer ISIT ()

An array of six interdigital thin film Pt-electrodes made by silicon technology, where the 1,5 Mym fingers are 0,8 Mym spaced, have been arranged perpendicular to the axis of silicon etched channels. The shape and the volume of the channels have been varied in order to study the flow conditions and to have several capabilities to immobilize biocomponents. The construction of the microflow system allows an easy exchange of enzyme coated electrode chips. Alternativly, the small flow chamber formed above the electrodes can be filled with immobilized enzymes. The following analytical procedures have been realized: - multielectrode measurement of redox molecules produced by enzymes (e.g. lactate oxidase or glucose oxidase) using a novel disigned multipotentiostat. - redox recycling of reversible electron mediators, which are formed in hydrolytic enzymatic reactions (e.g. by alkaline phosphatase), by applying different potentials to the interdigital electrodes. The obtained results are prese nted and discussed. The application of one time multiple measurements and the redox recyclization in flow systems is a new potential way for the enhancement of sensitivity and reproducibility of miniaturized biosensing devices.