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Kryokonservierung mit einem gas- oder dampffoermigen Kuehlmedium

Preservation of biological samples by freezing, comprises passing droplets through a cooling zone chilled by a gas or vapor, to be frozen and drop into a collection station for sorting into compartments.
 
: Fuhr, G.R.; Zimmermann, H.; Obergriesser, F.

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Frontpage ()

DE 2002-10237125 A: 20020813
DE 2002-10237125 A: 20020813
WO 2003-EP8055 A: 20030723
DE 10237125 A1: 20040311
A01N0001
German
Patent, Electronic Publication
Fraunhofer IBMT ()

Abstract
Es werden Verfahren zur Herstellung gefrorener Proben (1), insbesondere fuer die Kryokonservierung biologischer Proben,beschrieben, mit den Schritten: Bereitstellung von fluessigen Proben mit einem Tropfengenerator (10), Abkuehlung der Proben in einer Kuehleinrichtung (20, 21, 22) mit einem Kuehlmedium, bis die Proben zumindest an ihrer Oberflaeche gefrieren, und Sammlung der Proben in einer Aufnahmeeinrichtung (40), wobei die Proben in der Kuehleinrichtung (20, 21, 22) mit einem gas- oder dampffoermigen Kuehlmedium gefroren werden. Es werden auch Vorrichtungen, insbesondere fuer die Kryokonservierung biologischer Proben, beschrieben.

 

WO2004017732 A UPAB: 20040418 NOVELTY - Preservation of biological samples, by freezing, comprising passing droplets through a cooling zone chilled by a gas or vapor, to be frozen and drop into a collection station for sorting into compartments, is new. DETAILED DESCRIPTION - For the preservation of samples (1) by freezing, liquid samples are formed by a droplet generator (10) using a generator unit (12) and a dropper (11), as a pipette or a piezo electric unit or as a bubble jet. The droplets are frozen in a cooling station (20) with a cold zone (21) using a gas or vapor cooling medium (22), until at least the sample surfaces are frozen. The frozen samples are gathered at a collecting station (40) into separate compartments. The sample droplets pass down through the cooling station by gravity. USE - The system is used for freezing biological samples for preservation e.g. cells, cell groups or cell components, for use in biotechnology, medicine and pharmacology. ADVANTAGE - The samples are frozen without damage, for storage and preservation, with selective sorting and individual handling.

: http://publica.fraunhofer.de/documents/PP-1386.html