Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Expression of genes encoding matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in normal and diseased canine mitral valves

 
: Aupperle, H.; Thielebein, J.; Kiefer, B.; März, I.; Dinges, G.; Schoon, H.-A.; Schubert, A.

:

Journal of comparative pathology 140 (2009), No.4, pp.271-277
ISSN: 0021-9975
English
Journal Article
Fraunhofer IZI ()
tissue inhibitor of matrix metalloproteinase; matrix metalloproteinase; heart valve; dog

Abstract
The pathogenesis of canine chronic valvular disease (CVD) is not fully characterized. The present study investigates the expression of genes encoding matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in normal and diseased mitral valves (MVs). Samples from normal (n = 15) or diseased (n = 10) canine MVs were subject to real-time polymerase chain reaction (PCR) for quantification of mRNA encoding MMP-1, -2, -9 and -14 and TIMP-2, -3 and -4. In normal valves there was low expression of mRNA encoding MMP-2, -9 and -14 and TIMP-3. In the valves from dogs with CVD there was significantly increased transcription of mRNA encoding MMP-1 and -14 and TIMP-2, -3 and -4, but no elevation in mRNA encoding MMP-2 and -9. MMps and TIMPs are therefore likely to be involved in extracellular matrix metabolism in canine MVs and there are significant alterations in the expression of genes encoding these molecules during CVD.

: http://publica.fraunhofer.de/documents/N-94677.html