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Verfahren und Anordnung zum elektrischen Kontaktieren eines membranumhuellten Objekts mit einer Elektrode

Procedure for electrically contacting a membrane-covered object e.g. cells with an electrode for researching electric stimulation and repulsion of electric signals from the object, by arranging nanoneedles on a carrier of the electrode
 
: Gimsa, J.; Gimsa, U.; Fiedler, S.; Mueller, T.; Scheel, W.

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Frontpage ()

DE 102007019842 A: 20070425
DE 102007019842 A: 20070425
C12M0001
German
Patent, Electronic Publication
Fraunhofer IZM ()

Abstract
(A1) Die Erfindung bezieht sich u. a. auf ein Verfahren zum elektrischen Kontaktieren eines membranumhuellten Objekts (30) mit einer Elektrode (10, 100). Erfindungsgemaess ist vorgesehen, dass zum Kontaktieren mindestens eine Elektrode (100) mit einem leitfaehigen Traeger (110) verwendet wird, auf dem eine Vielzahl an Nanonadeln (120) angeordnet ist und auf dem benachbarte Nanonadeln zueinander einen Abstand aufweisen, der kleiner als die Groesse des Objekts ist, und dass das Objekt mit den Nanonadeln in Kontakt gebracht wird.

 

DE 102007019842 A1 UPAB: 20081217 NOVELTY - The procedure for electrically contacting a membrane-covered object e.g. biological cells (30) or tissues, with an electrode (10) for researching electric stimulation and repulsion of electric signals from the object, comprises arranging nanoneedles on a conductive carrier of the electrode. The needles adjoining on the carrier with one another have an interval that is smaller than the size of the object, which is in contact with the nanoneedles. The biological cell, biological tissue, liposome, lipid film or product with multilamellar construction is contacted as the object. DETAILED DESCRIPTION - The procedure for electrically contacting a membrane-covered object e.g. biological cells (30) or tissues, with an electrode (10) for researching electric stimulation and repulsion of electric signals from the object, comprises arranging nanoneedles on a conductive carrier of the electrode. The needles adjoining on the carrier with one another have an interval that is smaller than the size of the object, which is in contact with the nanoneedles. The biological cell, biological tissue, liposome, lipid film or product with multilamellar construction is contacted as the object. The nanoneedles are distributed on the carrier in a section by electrodes in stochastic manner and in another section. The nanoneedles are non-conductive or bad conductive as the carrier and possess a diameter of 10-1200 nm, and a length of 100-20 micrometer. A probe-tip with nanoneedle arrays is used as the electrode. The object is coupled on two electrodes provided with the nanoneedles. The cells are grown up on the electrode in contacting frame. The electrode forms a component of neuro-sensor chips. The nanoneedles form a nanolawn on the carrier. The nanolawn is produced under use of nanoimprint technology, semiconductor technology and/or electrolytic separation. USE - The procedure for electrically contacting a membrane-covered object e.g. biological cells or tissues, with an electrode for researching electric stimulation and repulsion of electric signals from the object. ADVANTAGE - The procedure ensures the electrical contacting of membrane-covered object with an electrode without damaging the object with high precision and to reach maximum low-impedance coupling between the cells and/or tissues and the conductive electrode.

: http://publica.fraunhofer.de/documents/N-91415.html