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Diamond nano-wires, a new approach towards next generation electrochemical gene sensor platforms

Diamant Nanodrähte, eine neue Möglichkeit für die Realisierung von elektrochemischen Gen-Sensorplattformen
: Nebel, C.E.; Yang, N.; Uetsuka, H.; Osawa, E.; Tokuda, N.; Williams, O.


Diamond and Related Materials 18 (2009), No.5-8, pp.910-917
ISSN: 0925-9635
European Conference on Diamond, Diamond-Like Materials, Carbon Nanotubes, Nitrides and Silicon Carbide (Diamond) <19, 2008, Sitges>
Journal Article, Conference Paper
Fraunhofer IAF ()
diamond; Diamant; gene-sensor; Gensensor; DNA; ultra-micro-electrode array; vertically aligned diamond nanowire

A novel bio-sensing platform is introduced by combination of a) geometrically controlled DNA bonding using vertically aligned diamond nano-wires and b) the superior electrochemical sensing properties of diamond as transducer material. Ultra-hard vertically aligned diamond nano-wires are electrochemically modified to bond phenyl linker-molecules to their tips which provide mesospacing for DNA molecules on the transducer. The nano-wires are generated by reactive ion etching of metallically boron doped atomically smooth single crystalline CVD diamond. Surface properties are characterized by atomic force, scanning electron and scanning tunneling microcopy. Electro- and bio-chemical sensor properties are investigated using cyclic and differential pulse voltammetry as well as impedance spectroscopy with Fe(CN)63-/4- as redox mediators which reveal sensitivities of 2 pM on 3 mm2 sensor areas and superior DNA bonding stability over 30 hybridization/denaturation cycles. The fabrication of "all diamond" ultra-micro-electrode (UME) arrays and multi-gene sensors are discussed taking into account the unique properties of diamond.