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In vitro femtosecond laser-assisted nanosurgery of porcine posterior capsule

: Toropygin, S.G.; Krause, M.; Riemann, I.; Seitz, B.; Mestres, P.; Ruprecht, K.W.; König, K.


Journal of cataract and refractive surgery 34 (2008), No.12, pp.2128-2132
ISSN: 0886-3350
Journal Article
Fraunhofer IBMT ()

PURPOSE: To investigate femtosecond laser-assisted nanosurgery of the posterior capsule in a prospective in vitro animal study. SETTING: Department of Ophthalmology and Eye Hospital, University of Saarland, Homburg, and Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany. METHODS: The posterior capsules of 12 porcine eyes were irradiated with a nonamplified 90 MHz near-infrared 750 nm titanium:sapphire femtosecond laser. Intratissue and superficial laser cuts of laser-ablated (5 capsules) and control (1 capsule) specimens were examined by femtosecond multiphoton laser scanning microscopy (MLSM) and transmission electron microscopy (TEM). RESULTS: Laser exposure time and pulse power determined the width of the lesions, which ranged from 0.69 mu m +/- 0.19 (SD) to 2.81 +/- 0.5 mu m. Both MLSM and TEM revealed minimal collateral alterations in the tissue surrounding the laser cuts. CONCLUSIONS: Nonamplified near-infrared femtosecond laser pulses at low pulse energies may be a promising strategy for precise lamellar noncontact nanosurgery of the posterior capsule, with minimal structural collateral damage to surrounding tissue. High-resolution MLSM offers 3-dimensional, noninvasive, nondestructive imaging at submicrometer resolution within seconds before and after ablation.