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Verfahren zur Anreicherung von Melanozyten mittels modifizierter Oberflaechen

Obtaining melanocytes from epidermal cell suspension and/or cell suspension from hair root, comprises cultivating the suspension in culture container, ensuring adherence of the melanocytes at container surface and obtaining the melanocytes
 
: Mertsching, H.; Schweizer, P.; Brunner, H.; Oehr, C.; Sciarratta, V.

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DE 102006057742 A: 20061207
DE 102006057742 A: 20061207
C12M0001
C12M0003
German
Patent, Electronic Publication
Fraunhofer IGB ()

Abstract
(A1) Die vorliegende Erfindung betrifft Verfahren zur Gewinnung von Melanozyten aus einer Zellsuspension, insbesondere aus einer epidermalen Zellsuspension, mittels eines Kulturgefaesses, wobei mindestens ein Teil der dem Kulturraum zugewandten Oberflaeche des Kulturgefaesses, insbesondere mittels eines Niederdruckplasmaverfahrens, modifiziert, insbesondere funktionalisiert, ist. Weiterhin betrifft die vorliegende Erfindung Kulturgefaesse, die, insbesondere mittels eines Niederdruckplasmaverfahrens, modifiziert, insbesondere funktionalisiert, sind und sich zur Gewinnung von Melanozyten eignen und die Verwendung solcher Kulturgefaesse zur Gewinnung von Melanozyten.

 

DE 102006057742 A1 UPAB: 20080815 NOVELTY - The method of obtaining melanocytes from an epidermal cell suspension and/or a cell suspension from a hair root, comprises cultivating the cell suspension in a culture container, ensuring adherence of the melanocytes present in the cell suspension at the culture container surface turned to the culture area and obtaining the adherence melanocytes. A part of culture container surface turned to the culture area is modified and functionalized by a low-pressure plasma procedure using acrylic acid, ethylene oxide, inert gas and/or reactive gas at a pressure of 0.5 bar for 10 minutes. DETAILED DESCRIPTION - The method of obtaining melanocytes from an epidermal cell suspension and/or a cell suspension from a hair root, comprises cultivating the cell suspension in a culture container, ensuring adherence of the melanocytes present in the cell suspension at the culture container surface turned to the culture area and obtaining the adherence melanocytes. A part of culture container surface turned to the culture area is modified and functionalized by a low-pressure plasma procedure using acrylic acid, ethylene oxide, inert gas and/or reactive gas at a pressure of 0.5 bar for 10 minutes. The cell suspension is a human-, a murine-, a bovine-, a canine-, a porcine- or feline- epidermal cell suspension. The cell suspension before cultivating in the culture container is pre-cultivated for 1-60 minutes in an unmodified culture container. The culture container or the surface of the culture container contains or consists of a plastic, hydrogel, silicon or glass. An INDEPENDENT CLAIM is included for a culture container for the obtaining of melanocytes. USE - Method of obtaining melanocytes from an epidermal cell suspension and/or a cell suspension from a hair root (claimed). ADVANTAGE - The procedure ensures simple, effective and economical production of cell culture with a large proportion of melanocytes or of pure melanocyte culture.

: http://publica.fraunhofer.de/documents/N-81995.html