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Multiphoton imaging of cardiovascular structures

: Schenke-Layland, K.; Riemann, I.; Ulrich, V.; Opitz, F.; Stock, U.A.; König, K.


Avrillier, S. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.; European Optical Society -EOS-:
Femtosecond laser applications in biology : 29 April 2004, Strasbourg, France
Bellingham, WA: SPIE, 2004 (SPIE Proceedings Series 5463)
ISBN: 0-8194-5386-2
Conference "Femtosecond Laser Applications in Biology" <2004, Strasbourg>
Conference Paper
Fraunhofer IBMT ()

Near infrared (NIR) femtosecond laser imaging systems represent a novel and very promising diagnostic technology for non-invasive cross-sectional analysis of living biological tissues. In this study 3D multiphoton imaging with submicron resolution has been performed for non-invasive analysis of living native and tissue-engineered (TE) heart valves and blood vessels. High-resolution autofluorescence and second harmonic generation (SHG) images of collagenous structures and elastic fibers were demonstrated using multiphoton excitation at two different wavelengths. Non-invasive optical sections have been obtained without the need of staining or embedding. The quality of the resulting three-dimensional images allowed exact differentiation between collagenous structures and elastic fibers. These experimental results are very encouraging for NIR femtosecond laser scanning microscopy as a useful tool for future non-destructive monitoring and characterization of vital and intact TE cardiovascular structures.