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Biologisierte Oberflächen für nachhaltige tribologische Anwendungen. Untersuchung zur Einbringung und in situ-Kultivierung von Pseudomonas fluorescens in technischen Materialien

: Wedemeyer, Tobias
: Harms, Carsten; Stübing, Dorothea

Bremerhaven, 2020, 94 pp.
Bremerhaven, Hochschule, Master Thesis, 2020
Master Thesis
Fraunhofer IFAM ()

The move away from fossil fuels will be one of the defining issues in the coming years and decades of the global community. Due to climate change, some areas, such as fossil fuels, are omnipresent, but the diversity of these areas extends far deeper. One of these less focused areas is the lubricant industry, which nevertheless accounts for a large, steadily increasing proportion. Vegetable oils and bio-lubricants offer widespread alternatives here, but so far they have not been a complete replacement, which is why this sub-area is still the subject of various research. For this reason, it was investigated in this work to use bacterial biofilms for tribological applications and to settle a self-sustaining and regulating biofilm on different micro-structured surfaces. For this purpose, a biofilm-forming, non-pathogenic strain was selected with Pseudomonas fluorescens (type strain DSM 50090) and its potential suitability was examined. Different cultivation conditions and surfaces were tested and optimized for this. With four different nutrient media, different nutrient additions and two different materials made of stainless steel and titanium, each with 5 different micro-structured surfaces, an attempt was made to achieve this goal. The resulting biofilm was examined via contact angle measurement, fluorescence microscopy, live-dead staining and macroscopic considerations of its vitality in a four-week main test under previously optimized conditions. Furthermore, the strain was transformed with a pGLO plasmid and the differences between marker gene and natural fluorescence were observed. In this way, conditions could be created to maintain a Pseudomonas fluorescens biofilm over four weeks on titanium and stainless steel, with differently structured sur-faces, partially completely vital, and thus lay the foundation for a wide range of further research on this topic. Finally, the results were evaluated, classified in the scientific con-text and extensive opportunities for further research were shown.