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Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis

: Godino, N.; Pfisterer, F.; Gerling, T.; Guernth-Marschner, C.; Duschl, C.; Kirschbaum, M.

Fulltext ()

LAB on a chip 19 (2019), No.24, pp.4016-4020
ISSN: 1473-0197
ISSN: 1473-0189
Journal Article, Electronic Publication
Fraunhofer IZI ()

With the advent of single-cell technologies comes the necessity for efficient protocols to process single cells. We combine dielectrophoresis with open source computer vision programming to automatically control the trajectories of single cells inside a microfluidic device. Using real-time image analysis, individual cells are automatically selected, isolated and spatially arranged.