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Perfusion chamber for cell tests with micropatterned surface

Perfusionskammer für Zelltests mit mikrotexturierten Oberflächen
: Jäger, M.; Rabenau, M.; Sonntag, F.; Poll, R.

Boenick, U. ; International Organization for Medical Physics -IOMP-; European Federation of Organizations in Medical Physics (EFOMP); German Society of Medical Physics (DGMP); German Society for Biomedical Engineering (DGBMT):
Medical Physics. Vol. 1 : Proceedings of the jointly held Congresses ICMP and BMT
Berlin: Schiele & Schön, 2005 (Biomedizinische Technik 50, Suppl. 1)
ISSN: 0939-4990
International Conference of Medical Physics (ICMP) <14, 2005, Nürnberg>
Biomedical Engineering Conference (BMT) <39, 2005, Nürnberg>
Conference Paper, Journal Article
Fraunhofer IWS ()
Perfusion; Oberflächenbearbeitung; Bioreaktor; Zellkultur; Mikrostrukturtechnik

Character and direction of cell growth can be influenced by localized surface modification. This is of great importance in both scientific and practical respects (cell micro arrays, tissue engineering). Laser-structuring of polymeric surfaces in microscale and nanoscale is of particular interest. Latest publications show that combining physical, chemical and topographical cues result in one combined biological effect. A modular bioreactor for perfusion cultures has been designed to support the analysis of these technologies. Its unique feature is a selective processing of the polystyrene cell contact surfaces before mounting the perfusion chamber. This preprocessing includes an isolated structuring and modification of the polymeric surface to adjust properties such as the concentration of nitrogen, surface tension, wetting etc. The design of the perfusion chamber encourages a reproducible culturing. It meets the special requirements of laboratory use and it makes possible the above mentioned preparations and analysis. In particular, the perfusion was optimized by extensive modelling in ANSYS-FLOTRAN.