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  4. Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform
 
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2006
Journal Article
Title

Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform

Abstract
L-DNA is the perfect mirror-image form of the naturally occurring d-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA-protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.
Author(s)
Hauser, N.C.  
Martinez, R.
Jacob, A.
Rupp, S.  
Hoheisel, J.D.
Matysiak, S.
Journal
Nucleic Acids Research  
Open Access
DOI
10.1093/nar/gkl671
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English
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
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