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Automated multichamber time-lapse videography for long-term in vivo observation of migrating cells

 
: Buhler, H.; Adamietz, R.; Abeln, T.; Diaz-Carballo, D; Nguemgo-Kouam, P.; Hero, T.; Adamietz, I.A.

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In vivo 31 (2017), No.3, pp.329-334
ISSN: 1791-7549
ISSN: 0258-851X
English
Journal Article

Abstract
Aim: To observe and document the migration of living cells by time-lapse videography, we constructed a lowbudget system based on a common inverted microscope. Materials and Methods: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37°C). Points of interest can be revisited in definable intervals with <1 μm repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. Results: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. Conclusion: This study demonstrates that useful videographic systems can be constructed at low cost.

: http://publica.fraunhofer.de/documents/N-590350.html