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Label-free and fluorescence photonic crystal biochips for early cancer biomarker detection

 
: Sinibaldi, A.; Danz, N.; Sepe, E.; Munzert, P.; Occhicone, A.; Allegretti, M.; Giordani, E.; Giacomini, P.; Michelotti, F.

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Danielli, A. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Frontiers in Biological Detection: from Nanosensors to Systems XI : 3-4 February 2019, San Francisco, California, United States
Bellingham, WA: SPIE, 2019 (Proceedings of SPIE 10895)
ISBN: 978-1-5106-2433-7
ISBN: 978-1-5106-2432-0
Paper 1089507, 7 pp.
Conference "Frontiers in Biological Detection - From Nanosensors to Systems" <11, 2019, San Francisco/Calif.>
Biophotonics, Biomedical Optics, and Imaging Conference (BIOS) <2019, San Francisco/Calif.>
Photonics West Conference <2019, San Francisco/Calif.>
English
Conference Paper
Fraunhofer IOF ()

Abstract
We report on the development of a biosensing platform that combines label-free and fluorescence based detection on disposable Bloch surface wave biochips. This system is applied to the detection of the HER2-neu/ErbB2 clinical biomarker related to breast cancer development. We first describe the design and fabrication of the BSW biochips as well as the principle of operation of the optical reading instrument. Then, the approaches for surface functionalization and immobilization of proteins for specific detection on the biochips are discussed. Finally, experimental results on a sandwich immunoassay for ErbB2 detection in cell lysates are presented.

: http://publica.fraunhofer.de/documents/N-569137.html