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Two-step production of neofructo-oligosaccharides using immobilized heterologous aspergillus terreus 1f-fructosyltransferase expressed in Kluyveromyces lactis and native Xanthophyllomyces dendrorhous G6-fructosyltransferase

: Burghardt, J.P.; Baas, M.; Gerlach, D.; Czermak, P.

Fulltext ()

Catalysts 9 (2019), No.8, Art. 673, 18 pp.
ISSN: 2073-4344
Journal Article, Electronic Publication
Fraunhofer IME ()

Fructo-oligosaccharides (FOS) are prebiotic low-calorie sweeteners that are synthesized by the transfer of fructose units from sucrose by enzymes known as fructosyltransferases. If these enzymes generate β-(2,6) glycosidic bonds, the resulting oligosaccharides belong to the neoseries (neoFOS). Here, we characterized the properties of three different fructosyltransferases using a design of experiments approach based on response surface methodology with a D-optimal design. The reaction time, pH, temperature, and substrate concentration were used as parameters to predict three responses: The total enzyme activity, the concentration of neoFOS and the neoFOS yield relative to the initial concentration of sucrose. We also conducted immobilization studies to establish a cascade reaction for neoFOS production with two different fructosyltransferases, achieving a total FOS yield of 47.02 ± 3.02%. The resulting FOS mixture included 53.07 ± 1.66 mM neonystose (neo-GF3) and 20.8 ± 1.91 mM neo-GF4.