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Automated Microfluidic Cell Culture of Stem Cell Derived Dopaminergic Neurons

 
: Kane, Kaild; Lucumi Moreno, Edinson; Hachi, Siham; Walter, Moriz; Jarazo, Javier; Oliveira, Miguel; Hankemeier, Thomas; Vulto, Paul; Schwamborn, Jens; Thoma, Martin; Fleming, Ronan

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Fulltext urn:nbn:de:0011-n-5377093 (2.0 MByte PDF)
MD5 Fingerprint: 976fe4d84fee9aa58bedffd72b21ebbf
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Created on: 26.3.2019


Scientific Reports 9 (2019), Art. 1796, 12 pp.
ISSN: 2045-2322
English
Journal Article, Electronic Publication
Fraunhofer IPA ()
automatisierte Zellkultur; Neurobiologie; Neurowissenschaft; Parkinson-Krankheit

Abstract
Parkinson’s disease is a slowly progressive neurodegenerative disease characterised by dysfunction and death of selectively vulnerable midbrain dopaminergic neurons and the development of human in vitro cellular models of the disease is a major challenge in Parkinson’s disease research. We constructed an automated cell culture platform optimised for long-term maintenance and monitoring of different cells in three dimensional microfluidic cell culture devices. The system can be flexibly adapted to various experimental protocols and features time-lapse imaging microscopy for quality control and electrophysiology monitoring to assess cellular activity. Using this system, we continuously monitored the differentiation of Parkinson’s disease patient derived human neuroepithelial stem cells into midbrain specific dopaminergic neurons. Calcium imaging confirmed the electrophysiological activity of differentiated neurons and immunostaining confirmed the efficiency of the differentiation protocol. This system is the first example of an automated Organ-on-a-Chip culture and has the potential to enable a versatile array of in vitro experiments for patient-specific disease modelling.

: http://publica.fraunhofer.de/documents/N-537709.html