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  4. Automated Microfluidic Cell Culture of Stem Cell Derived Dopaminergic Neurons
 
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2019
Journal Article
Title

Automated Microfluidic Cell Culture of Stem Cell Derived Dopaminergic Neurons

Abstract
Parkinson's disease is a slowly progressive neurodegenerative disease characterised by dysfunction and death of selectively vulnerable midbrain dopaminergic neurons and the development of human in vitro cellular models of the disease is a major challenge in Parkinson's disease research. We constructed an automated cell culture platform optimised for long-term maintenance and monitoring of different cells in three dimensional microfluidic cell culture devices. The system can be flexibly adapted to various experimental protocols and features time-lapse imaging microscopy for quality control and electrophysiology monitoring to assess cellular activity. Using this system, we continuously monitored the differentiation of Parkinson's disease patient derived human neuroepithelial stem cells into midbrain specific dopaminergic neurons. Calcium imaging confirmed the electrophysiological activity of differentiated neurons and immunostaining confirmed the efficiency of the differentiation protocol. This system is the first example of an automated Organ-on-a-Chip culture and has the potential to enable a versatile array of in vitro experiments for patient-specific disease modelling.
Author(s)
Kane, Kaild
Universität Luxemburg / LCSB
Lucumi Moreno, Edinson
Universität Luxemburg / LCSB / Universität Leiden / Division of Systems Biomedicine and Pharmacology
Hachi, Siham
Universität Luxemburg / LCSB
Walter, Moriz
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA  
Jarazo, Javier
Universität Luxemburg / LCSB
Oliveira, Miguel
Universität Luxemburg / LCSB
Hankemeier, Thomas
Universität Leiden / Division of Systems Biomedicine and Pharmacology
Vulto, Paul
MIMETAS
Schwamborn, Jens
Universität Luxemburg / LCSB
Thoma, Martin  
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA  
Fleming, Ronan
Universität Luxemburg / LCSB / Universität Leiden / Division of Systems Biomedicine and Pharmacology
Journal
Scientific Reports  
Open Access
File(s)
Download (2.02 MB)
Rights
CC BY 4.0: Creative Commons Attribution
DOI
10.24406/publica-r-257043
10.1038/s41598-018-34828-3
Language
English
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA  
Keyword(s)
  • automatisierte Zellkultur

  • Neurobiologie

  • Neurowissenschaft

  • Parkinson-Krankheit

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