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Dielectrophoretic stretching of DNA

: Laux, E.-M.; Bier, F.F.; Hölzel, R.


Zuccheri, G.:
DNA Nanotechnology. Methods and Protocols : 2nd edition 2018
New York: Springer Science+Business Media, 2018 (Methods in molecular biology 1811)
ISBN: 978-1-4939-8581-4 (Print)
ISBN: 978-1-4939-8582-1 (Online)
Book Article
Fraunhofer IZI ()

The spatial control of DNA and of self-assembled DNA constructs is a prerequisite for the preparation of DNA-based nanostructures and microstructures and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved λ-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and align them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.