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  4. Label-free detection of angiogenesis biomarkers using Bloch surface waves on one dimensional photonic crystals
 
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2015
Conference Paper
Title

Label-free detection of angiogenesis biomarkers using Bloch surface waves on one dimensional photonic crystals

Abstract
We describe the design and fabrication of biochips based on one dimensional photonic crystals supporting Bloch surface waves for label-free optical biosensing. The planar stacks of the biochips are composed of silica, tantala and titania that were deposited using plasma ion assisted evaporation under high vacuum conditions. The biochip surfaces were functionalized by silanization and appropriate fluidic cells were designed to operate in an automated platform. An angularly resolved optical sensing apparatus was assembled to carry out the sensing studies. The angular operation is obtained by a focused laser beam at a fixed wavelength and detection of the angular reflectance spectrum by means of an array detector. Practical application of the sensor was demonstrated by detecting a specific glycoprotein, Angiopoietin 2, that is involved in angiogenesis and inflammation processes. The protocol used for the label-free detection of Angiopoietin 2 is described and the results of an exemplary assay are given, confirming that an efficient detection can be achieved.
Author(s)
Sinibaldi, A.
Universität La Sapienza
Anopchenko, Aleksei
Universität La Sapienza
Occhicone, Agostino
Universität La Sapienza
Michelotti, Francesco
Universität La Sapienza
Danz, Norbert  
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Munzert, Peter  
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Schmieder, Stefan
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS  
Sonntag, Frank  orcid-logo
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS  
Chandrawati, Rona
South Kensington Campus
Rana, Subinoy
South Kensington Campus
Stevens, Molly M.
South Kensington Campus
Napione, Lucia
Universität Turin
Mainwork
1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOfIM 2015  
Conference
Workshop on Nanotechnology in Instrumentation and Measurement (NANOFIM) 2015  
DOI
10.1109/NANOFIM.2015.8425342
Language
English
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS  
Keyword(s)
  • biophotonic

  • Bloch surface wave

  • ellipsometry

  • label-free biosensor

  • optical biosensing

  • photonic crystal

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