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Ein neuartiger Assay zur Messung chemotaktischer Migration in Echtzeit

A novel assay for the measurement of chemotactic migration in real time
 
: O’Clair, L.; Ellinger, B.; Kalmer, M.; Szybut, C.; Borsetti, Y.

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Biospektrum 23 (2017), No.2, pp.171-173
ISSN: 0947-0867
ISSN: 1868-6249
German, English
Journal Article
Fraunhofer IME ()

Abstract
Traditional techniques for studying chemotaxis, such as Boyden chamber assays, are limited due to their endpoint nature and inability to visualize cells. A novel technique is presented that generates kinetic, automated, image-based measurements and movies of chemotaxis in a reproducible 96-well format. This technique offers the advantages of visual confirmation of migration and morphology, reduced cell requirement and the use of biologically relevant surface substrates, with or without fluorescent labels.

: http://publica.fraunhofer.de/documents/N-503196.html