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Comparative proteomic analysis of hepatic effects induced by nanosilver, silver ions and nanoparticle coating in rats

 
: Juling, Sabine; Böhmert, Linda; Lichtenstein, Dajana; Oberemm, Axel; Creutzenberg, Otto H.; Thünemann, Andreas F.; Braeuning, Albert; Lampen, Alfonso

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Food and Chemical Toxicology : FCT 113 (2018), pp.255-266
ISSN: 0278-6915
ISSN: 1873-6351
English
Journal Article
Fraunhofer ITEM ()
silver nanoparticles NM-300; silver acetate; coating; 2-D gel MALDI-TOF MS / MS; in vivo; Proteomics

Abstract
The presence of nano-scaled particles in food and food-related products has drawn attention to the oral uptake of nanoparticles and their interactions with biological systems. In the present study, we used a toxico proteomics approach to allow for the untargeted experimental identification and comparative analysis of cellular responses in rat liver after repeated-dose treatment with silver nanoparticles, ions, and the coating matrix used for particle stabilization. The proteomic analysis revealed treatment-related effects caused by exposure to silver in particulate and ionic form. Both silver species induced similar patterns of signaling and metabolic alterations. Silver-induced cellular alterations comprised, amongst others, proteins involved in metal homeostasis, oxidative stress response, and energy metabolism. However, we discovered that secondary nano-scaled structures were formed from ionic silver. Furthermore, also the coating matrix alone gave rise to the formation of nano-scaled particles. The present data confirm, complement, and extend previous knowledge on silver toxicity in rodent liver by providing a comprehensive proteomic data set. The observation of secondary particle formation from non-particle controls underlines the difficulties in separating particle-, ion-, and matrix coating-related effects in biological systems. Awareness of this issue will support proper evaluation of nanotoxicology-related data in the future.

: http://publica.fraunhofer.de/documents/N-497560.html