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Involvement of topoisomerase II inhibition in clastogenic activity of trans-b-Nitrostyrene

 
: Ziemann, C.; Krauß, S.; Brockmeyer, H.; Leonhardt, U.; Steinfelder, H.J.

Deutsche Gesellschaft für Experimentelle und Klinische Pharmakologie und Toxikologie -DGPT-:
47th Spring Meeting 2006. Deutsche Gesellschaft für Experimentelle und Klinische Pharmakologie und Toxikologie. Abstracts : 4 - 6 April 2006, Mainz, Germany
Berlin: Springer, 2006 (Naunyn-Schmiedeberg's Archives of Pharmacology 372.2006, Suppl.1)
ISSN: 0028-1298
Abstract 441
Deutsche Gesellschaft für Experimentelle und Klinische Pharmakologie und Toxikologie (Spring Meeting) <47, 2006, Mainz>
English
Abstract
Fraunhofer ITEM ()

Abstract
Trans-b-nitrostyrene (b-NS) is used as chain stopper in styrene type polymerization reactions. Interestingly, NS displays promising anti-tumour potential, due to its profound pro-apoptotic properties. We have previously demonstrated that b-NS-triggered formation of DNA strand breaks (SBs), particularly double-strand breaks (DSBs), participates in apoptosis induction. DSB-formation, as determined in the neutral comet-assay, was comparable to that of the topoisomerase II (TopoII) poison etoposide. Therefore, we investigated whether b-NS-induced DNA-damage is also based on inhibition of the breakage-resealing process of TopoII action. b-NS, in contrast to ethyl methane sulfonate (EMS), induced no DNA-damage in an acellular comet-assay, indeed pointing to an enzyme-linked process of DNA-breakage. Furthermore, induction of SBs by b-NS-treatment was strongly related to cell proliferation, as highly proliferating L5178YTK+/- mouse lymphoma cells were signifi

: http://publica.fraunhofer.de/documents/N-48522.html