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2018
Journal Article
Titel
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis
Abstract
Staphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine(PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW(Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G(F-Br = 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applications.
Author(s)
Hernandez-Montelongo, J.
Departamento de FĆsica Aplicada, Instituto de FĆsica Gleb Wataghin, Universidade Estadual de Campinas, 13083-859 Campinas, Brazil & Departamento de Ciencias MatemĆ”ticas y FĆsicas, Facultad de IngenierĆa, Universidad CatĆ³lica de Temuco 4813302 Temuco, Chile & NĆŗcleo de InvestigaciĆ³n en Bioprodructos y Materiales Avanzados (BioMa), Facultad de IngenierĆa, Universidad CatĆ³lica de Temuco, 4781312, Temuco, Chile
Corrales UreƱa, Y.R.
Laboratorio Nacional de NanotecnologĆa, Centro Nacional de Alta TecnologĆa, 1174- 1200, San JosĆ©, Costa Rica
Machado, D.
Faculdade de CiĆŖncias FarmacĆŖuticas, Universidade Estadual de Campinas, 13083-871 Campinas, Brazil
Lancellotie, M.
Faculdade de CiĆŖncias FarmacĆŖuticas, Universidade Estadual de Campinas, 13083-871 Campinas, Brazil
Lisboa-Filho, P.N.
Departamento de FĆsica, Faculdade de CiĆŖncias, Universidade Estadual Paulista, 17033-360 Bauru, Brasil