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Photoinduced cross-linking of short furan-modified DNA on surfaces

: Montes, C.V.; Memczak, H.; Gyssels, E.; Torres, T.; Madder, A.; Schneider, R.J.


Langmuir. The ACS journal of surfaces and colloids 33 (2017), No.5, pp.1197-1201
ISSN: 0743-7463
ISSN: 1520-5827
European Commission EC
FP7-PEOPLE; 316975; SO2S
"The Singlet Oxygen Strategy: sustainable oxidation procedures for applications in material science, synthesis, wastewater treatment, diagnostics and therapeutics"
Journal Article
Fraunhofer IZI ()

We report for the first time the formation of site specific interstrand cross p-linked (ICL) surface -immobilized furanmodified DNA duplexes-via singlet oxygen. 102, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonudeotides.