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Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium

: Inprakhon, Pranee; Wongthongdee, Natcha; Amornsakchai, Taweechai; Pongtharankul, Thunyarat; Sunintaboon, Panya; Wiemann, Lars O.; Durand, Alain; Sieber, Volker


Journal of biotechnology 259 (2017), pp.182-190
ISSN: 0168-1656
ISSN: 1389-0352
ISSN: 1873-4863
Journal Article
Fraunhofer IGB ()
lipase; sucrose; sucrose ester; transesterification

Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7. M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2. M sucrose monoester within 1. h at 50. °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product.